pmid: 31721526,   nlmid 101566618,  doi:10.22074/cellj.2020.6738

Designing A Transgenic Chicken: Applying New Approaches toward A Promising Bioreactor.


Bahrami, Salahadin Amiri-Yekta, Amir Daneshipour, Abbas Jazayeri, Seyedeh Hoda Mozdziak, Paul Edward Sanati, Mohammad Hossein Gourabi, Hamid

Vol. 22, Issue 22, Jul.2020

Cell journal (Cell J),  ISSN 2228-5806

Abstract

Specific developmental characteristics of the chicken make it an attractive model for the generation of transgenic organisms. Chicken possess a strong potential for recombinant protein production and can be used as a powerful bioreactor to produce pharmaceutical and nutritional proteins. Several transgenic chickens have been generated during the last two decades via viral and non-viral transfection. Culturing chicken primordial germ cells (PGCs) and their ability for germline transmission ushered in a new stage in this regard. With the advent of CRISPR/Cas9 system, a new phase of studies for manipulating genomes has begun. It is feasible to integrate a desired gene in a predetermined position of the genome using CRISPR/Cas9 system. In this review, we discuss the new approaches and technologies that can be applied to generate a transgenic chicken with regards to recombinant protein productions.


MESH Headings


Article Keywords

CRISPR/Cas9 | Chickens | Ovalbumin | Recombinant Protein | Transgenes |


Chemical

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