pmid: 31721530,   nlmid 101566618,  doi:10.22074/cellj.2020.6555

Evaluation of The Expression Levels of Three Long Non-Coding RNAs in Multiple Sclerosis.

Moradi, Afshin Rahimi Naiini, Mahdis Yazdanpanahi, Nasrin Tabatabaeian, Hossein Nabatchian, Fariba Baghi, Masoud Azadeh, Mansoureh Ghaedi, Kamran

Vol. 22, Issue 22, Jul.2020

Cell journal (Cell J),  ISSN 2228-5806


Multiple sclerosis (MS) is a chronic disorder involving both inflammatory and neurodegenerative responses. Long non-coding RNAs (lncRNAs) have been had an emerging role as the biomarkers of different disorders, including autoimmune diseases. Previous studies have shown that NR_003531.3 (MEG3a), AC000061.1_201, and AC007182.6 play a role in the pathogenesis of human autoimmune diseases. However, the potential significance of these lncRNAs, as the diagnostic biomarkers of MS, has not been studied yet. We aimed to quantitatively evaluate the expression levels of NR_003531.3, AC000061.1_201, and AC007182.6 in peripheral blood samples of MS patients in comparison with healthy controls. In this case-control study, the blood samples from 20 MS patients and 10 healthy controls were collected. Total RNA was extracted, and the expression levels of three selected lncRNAs were quantitatively measured using the quantitative real time-polymerase chain reaction (qRT-PCR) method. We detected a significant down-regulation in the expression of NR_003531.3 in MS patients, while no marked changes were observed in the expression of AC000061.1_201 and AC007182.6 in patients compared with controls. Based on the receiver operating characteristic (ROC) curve analysis, NR_003531.3 could discriminate MS patients from healthy subjects effectively. Regarding the prognosis of MS patients, NR_003531.3 is significantly and inversely correlated with the expanded disability status scale (EDSS). The potential role of NR_003531.3 lncRNA as a diagnostic biomarker to distinguish MS patients is proposed. Prognostically, NR_003531.3 correlates with lower disability rates in MS patients.

MESH Headings

Article Keywords

Autoimmune Disease | Gene Expression Profiling | Long Non-coding RNA | Multiple Sclerosis | Neurodegenerative Disease |


Similar Articles

Repetitive element hypermethylation in multiple sclerosis patients.
Neven, K Y Piola, M Angelici, L Cortini, F Fenoglio, C Galimberti, D Pesatori, A C Scarpini, E Bollati, V
BMC genetics ,vol(17), issue(1),  Jun.2016

Integrative Analysis of lncRNAs in Th17 Cell Lineage to Discover New Potential Biomarkers and Therapeutic Targets in Autoimmune Diseases.
Teimuri, Shohreh Hosseini, Aref Rezaenasab, Ahmad Ghaedi, Kamran Ghoveud, Elahe Etemadifar, Masoud Nasr-Esfahani, Mohammad Hossein Megraw, Timothy L
Molecular therapy. Nucleic acids ,vol(12), issue(),  Sep.2018

Integrative analysis of OIP5-AS1/HUR1 to discover new potential biomarkers and therapeutic targets in multiple sclerosis.
Gharesouran, Jalal Taheri, Mohammad Sayad, Arezou Mazdeh, Mehrdokht Omrani, Mir Davood
Journal of cellular physiology ,vol(234), issue(10),  Aug.2019

Down-Regulation of lncRNA-AK001085 and its Influences on the Diagnosis of Ankylosing Spondylitis.
Li, Xiang Chai, Wei Zhang, Guoqiang Ni, Ming Chen, Jiying Dong, Jiyuan Zhou, Yonggang Hao, Libo Bai, Yang Wang, Yan
Medical science monitor : international medical journal of experimental and clinical research ,vol(23), issue(),  Jan.2017

Differential Plasma Expression Profiles of Long Non-Coding RNAs Reveal Potential Biomarkers for Systemic Lupus Erythematosus.
Wu, Guo-Cui Hu, Yan Guan, Shi-Yang Ye, Dong-Qing Pan, Hai-Feng
Biomolecules ,vol(9), issue(6),  May.2019

HOTAIR Long Noncoding RNA is not a Biomarker for Acute Myeloid Leukemia (AML) in Iranian Patients
Sayad, Arezou Hajifathali, Abbas Hamidieh, Amir Ali Roshandel, Elham Taheri, Mohammad
Asian Pacific journal of cancer prevention : APJCP ,vol(18), issue(6),  Jun.2017

Expression Analysis of Long Non-coding RNAs in the Blood of Multiple Sclerosis Patients.
Eftekharian, Mohammad Mahdi Ghafouri-Fard, Soudeh Soudyab, Mohammad Omrani, Mir Davood Rahimi, Mahnoosh Sayad, Arezou Komaki, Alireza Mazdeh, Mehrdokht Taheri, Mohammad
Journal of molecular neuroscience : MN ,vol(63), issue(3-4),  Dec.2017

HULC and Linc00152 Act as Novel Biomarkers in Predicting Diagnosis of Hepatocellular Carcinoma.
Li, Jun Wang, Xiaochen Tang, Junwei Jiang, Runqiu Zhang, Wenjie Ji, Jie Sun, Beicheng
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology ,vol(37), issue(2),  

Serum HOTAIR as a novel diagnostic biomarker for esophageal squamous cell carcinoma.
Wang, Wenjian He, Xiaotian Zheng, Zehua Ma, Xiaofan Hu, Xueting Wu, Duoguang Wang, Minghui
Molecular cancer ,vol(16), issue(1),  Apr.2017

Long Non-Coding RNA: Potential Diagnostic and Therapeutic Biomarker for Major Depressive Disorder.
Cui, Xuelian Sun, Xinyang Niu, Wei Kong, Lingming He, Mingjun Zhong, Aifang Chen, Shengdong Jiang, Kunhong Zhang, Liyi Cheng, Zaohuo
Medical science monitor : international medical journal of experimental and clinical research ,vol(22), issue(),  Dec.2016

Medline data is available here.

The Search Enterprise

Contact Us