pmid: 31721532,   nlmid 101566618,  doi:10.22074/cellj.2020.6543

A Comparative Study of HOTAIR Expression in Breast Cancer Patient Tissues and Cell Lines.


Arshi, Asghar Raeisi, Farzaneh Mahmoudi, Esmaeil Mohajerani, Fatemeh Kabiri, Hamidreza Fazel, Razieh Zabihian-Langeroudi, Maedeh Jusic, Amela

Vol. 22, Issue 22, Jul.2020

Cell journal (Cell J),  ISSN 2228-5806

Abstract

Recent data suggest that increased levels of the HOTAIR long non-coding RNA (lncRNA) are involved in the development of various types of malignancy, including breast cancer. The aim of present study was to investigate HOTAIR lncRNA expression profile in breast cancer (BC) patients and cell lines. In this experimental study, expression level of HOTAIR lncRNA was evaluated in BC and normal tissues of 15 patients as well as MDA-MB-231, MCF-7 and MCF-10A cell lines, using quantitative reversetranscription polymerase chain reaction (qRT-PCR). HOTAIR lncRNA expression levels were estimated using 2-??Ct method. Further, receiver operating characteristic (ROC) curve analysis was done to evaluate the selected lncRNA diagnostic potential. The Cox's proportional hazards regression model was performed to evaluate the predictive value of this lncRNA level in BC patients. The results of present study demonstrated no signi?cant difference in the expression of HOTAIR lncRNA in MCF7 and MDA-MB-231 cancer cell lines compared to MCF-10A as normal cell line (P>0.05). However, we observed a signi?cantly increase in the expression of HOTAIR in BC patients compared to normal tissues (P<0.001). Signi?cant associations were found between gene expression and tumour size and margin. We found 91.1% sensitivity and 95.7% specificity of circulating HOTAIR with an area under the ROC curve of 0.969. The Kaplan-Meier analysis indicated significant correlation between HOTAIR expression and overall survival. This study demonstrated that expression of HOTAIR is increased in BC and might be associated with its progression. According to these findings, HOTAIR expression could be proposed as biomarkers for BC early diagnosis and prognosis.


MESH Headings


Article Keywords

Breast Cancer | Cell Line | HOTAIR lncRNA | Quantitative Reverse-Transcription Polymerase Chain Reaction |


Chemical

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